Accuracy and Precision of HDL Measurement With the Precipitation Method and LDL Measurement With the Friedewald Method Compared to the Enzymatic Method Using a Chemistry Analyzer

Abstract

HDL (High Density Lipoprotein) is a lipoprotein with high density that transports LDL (Low Density Lipoprotein) from peripheral blood vessels to the liver. LDL, a cholesterol-rich lipoprotein, can cause health issues if levels are too high. HDL levels can be measured by the precipitation method, while LDL levels are measured by the Friedewald method. To ensure these methods' feasibility, their accuracy and precision must be known. Accuracy refers to how closely examination results match the actual value, while precision refers to the consistency of repeated results. This study aimed to assess the accuracy and precision of HDL measurement by the precipitation method and LDL measurement by the Friedewald method compared to the enzymatic method using a chemistry analyzer. A descriptive analytic approach was used, with research conducted from December 2023 to May 2024 at RSUD Haji Provinsi Jawa Timur, East Java Province, and Farmalab Laboratory. The study involved 35 samples for accuracy and 2 for precision, analyzed using Microsoft Excel. Results showed that the HDL precipitation method had an accuracy of 107%, while the Friedewald method for LDL had an accuracy of 95%. Precision (CV) for HDL was 4% for both samples, and for LDL, it was 1% and 2% in the first and second samples, respectively. Both methods' accuracy and precision fall within acceptable limits.