Potential Combination of Pomelo Peel and Stevia Leaves Powder on The Stability of Red Blood Cell Membranes in Vitro

Abstract

Inflammation is a local proteactive response caused by damage to tissue because damaging chemicals, physical or microbiological trauma. Chronic excessive inflammatory reactions can also give rise to several diseases such as damage to tissues, resulting in damaging reactive radicals and autoimmune diseases. The research was conducted at Chemistry Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar. in this study the stability of red blood cells as the dependent variable and the variation of pomelo peel and stevia leaves powder as the independent variable which will affect the results of the stability of red blood cells. Pomelo peel and stevia leaves are dried in an oven at 50°C and then mashed into powder. The combination of pomelo peel and stevia leaves powder was made variation I, variation II and variation III with a different composition of pomelo peel and stevia leaves powder and then the absorbance of the sample was measured using a UV-Vis Spectrophotometer. This study was designed with a true experimental design method with complete randomized design, with testing the stability of red blood cell membranes tested in vitro.  Anti-inflammatory activity data were analyzed using ANNOVA's One Way SPSS (Statistical Package for Social Science) test followed by the Mann-Whitney test. In phytochemical tests, the combination of pomelo peel and stevia leaves powder is positive for secondary metabolite compounds, namely flavonoids, alkaloids, tannins and saponins. The results showed that the average percentage of stability of red blood cell membranes was variation I (99.05%), variation II (98.72%), variation III (98.62%) and positive control (99.64%). So, it can be concluded that in vitro test combination of pomelo peel and stevia leaves powder has the potential to maintain red blood cell stability.